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Sequence-specific inhibition of microRNA- and siRNA-induced RNA silencing

Item Type:Article
Title:Sequence-specific inhibition of microRNA- and siRNA-induced RNA silencing
Creators Name:Meister, G. and Landthaler, M. and Dorsett, Y. and Tuschl, T.
Abstract:A large number of miRNAs have recently been discovered in plants and animals. Development of reverse genetic approaches that act to inhibit microRNA function would facilitate the study of this new class of noncoding RNA. Here we show that 2'-O-methyl oligoribonucleotides, but not 2'-deoxyoligonucleotides specifically inactivate the RNAi activity associated with miRNA-protein complexes in human cell extracts as well as in cultured human cells.
Keywords:RNA Interference, miRNA, miRNP, Posttranscriptional Gene Regulation, Translational Regulation, mRNA Degradation
Source:RNA
ISSN:1355-8382
Publisher:Cold Spring Harbor Laboratory Press (U.S.A.)
Volume:10
Number:3
Page Range:544-550
Date:March 2004
Official Publication:https://doi.org/10.1261/rna.5235104
PubMed:View item in PubMed

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