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Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs

Item Type:Review
Title:Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs
Creators Name:Meister, G. and Landthaler, M. and Patkaniowska, A. and Dorsett, Y. and Teng, G. and Tuschl, T.
Abstract:Argonaute proteins associate with small RNAs that guide mRNA degradation, translational repression, or a combination of both. The human Argonaute family has eight members, four of which (Ago1 through Ago4) are closely related and coexpressed in many cell types. To understand the biological function of the different Ago proteins, we set out to determine if Ago1 through Ago4 are associated with miRNAs as well as RISC activity in human cell lines. Our results suggest that miRNAs are incorporated indiscriminately of their sequence into Ago1 through Ago4 containing microRNPs (miRNPs). Purification of the FLAG/HA-epitope-tagged Ago containing complexes from different human cell lines revealed that endonuclease activity is exclusively associated with Ago2. Exogenously introduced siRNAs also associate with Ago2 for guiding target RNA cleavage. The specific role of Ago2 in guiding target RNA cleavage was confirmed independently by siRNA-based depletion of individual Ago members in combination with a sensitive positive-readout reporter assay.
Keywords:Binding Sites, Drosophila Proteins, Gene Silencing, Reporter Genes, Hela Cells, MicroRNAs, Protein Biosynthesis, RNA, Antisense RNA, Messenger RNA, Small Interfering RNA, RNA-Induced Silencing Complex, Ribonucleoproteins
Source:Molecular Cell
Publisher:Cell Press
Page Range:185-197
Date:23 July 2004
Official Publication:https://doi.org/10.1016/j.molcel.2004.07.007
PubMed:View item in PubMed

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