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Endogenous MHC class II processing of a viral nuclear antigen after autophagy

Item Type:Article
Title:Endogenous MHC class II processing of a viral nuclear antigen after autophagy
Creators Name:Paludan, C. and Schmid, D. and Landthaler, M. and Vockerodt, M. and Kube, D. and Tuschl, T. and Muenz, C.
Abstract:CD4+ T cells classically recognize antigens that are endocytosed and processed in lysosomes for presentation on major histocompatibility complex (MHC) class II molecules. Here, endogenous Epstein-Barr virus nuclear antigen 1 (EBNA1) was found to gain access to this pathway by autophagy. On inhibition of lysosomal acidification, EBNA1, the dominant CD4+ T cell antigen of latent Epstein-Barr virus infection, slowly accumulated in cytosolic autophagosomes. In addition, inhibition of autophagy decreased recognition by EBNA1-specific CD4+ T cell clones. Thus, lysosomal processing after autophagy may contribute to MHC class II-restricted surveillance of long-lived endogenous antigens including nuclear proteins relevant to disease.
Keywords:Antigen Presentation, Autophagy, B-Lymphocytes, CD4-Positive T-Lymphocytes, Cell Line, Chloroquine, Epstein-Barr Virus Nuclear Antigens, Histocompatibility Antigens Class II, Hydrogen-Ion Concentration, Lysosomes, Microsomes, Phagosomes, Proteasome Endopeptidase Complex, Transfection, Animals
Source:Science
ISSN:0036-8075
Publisher:American Association for the Advancement of Science (U.S.A.)
Volume:307
Number:5709
Page Range:593-596
Date:28 January 2005
Official Publication:https://doi.org/10.1126/science.1104904
PubMed:View item in PubMed

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