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Identification of novel argonaute-associated proteins

Item Type:Article
Title:Identification of novel argonaute-associated proteins
Creators Name:Meister, G. and Landthaler, M. and Peters, L. and Chen, P.Y. and Urlaub, H. and Luehrmann, R. and Tuschl, T.
Abstract:RNA silencing processes are guided by small RNAs known as siRNAs and microRNAs (miRNAs) . They reside in ribonucleoprotein complexes, which guide the cleavage of complementary mRNAs or affect stability and translation of partial complementary mRNAs . Argonaute (Ago) proteins are at the heart of silencing effector complexes and bind the single-stranded siRNA and miRNA . Our biochemical analysis revealed that Ago2 is present in a pre-miRNA processing complex that is able to transfer the miRNA into a target-mRNA cleaving complex. To gain insight into the function and composition of RNA silencing complexes, we purified Ago1- and Ago2-containing complexes from human cells. Several known Ago1- and/or Ago2-associated proteins including Dicer were identified, but also two novel factors, the putative RNA helicase MOV10, and the RNA recognition motif (RRM)-containing protein TNRC6B/KIAA1093. The new proteins localize, similar to Ago proteins, to mRNA-degrading cytoplasmic P bodies, and they are functionally required to mediate miRNA-guided mRNA cleavage.
Keywords:Northern Blotting, Western Blotting, Cell Line, Eukaryotic Initiation Factors, Fluorescent Antibody Technique, Intracellular Signaling Peptides and Proteins, Mass Spectrometry, MicroRNAs, Oligonucleotides, Peptide Initiation Factors, RNA Helicases, Messenger RNA, Small Interfering RNA, RNA-Induced Silencing Complex, Reverse Transcriptase Polymerase Chain Reaction, Ribonuclease III
Source:Current Biology
Publisher:Cell Press
Page Range:2149-2155
Date:6 December 2005
Official Publication:https://doi.org/10.1016/j.cub.2005.10.048
PubMed:View item in PubMed

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