A mammalian microRNA expression atlas based on small RNA library sequencing

Item Type:	Article
Title:	A mammalian microRNA expression atlas based on small RNA library sequencing
Creators Name:	Landgraf, P. and Rusu, M. and Sheridan, R. and Sewer, A. and Iovino, N. and Aravin, A. and Pfeffer, S. and Rice, A. and Kamphorst, A.O. and Landthaler, M. and Lin, C. and Socci, N.D. and Hermida, L. and Fulci, V. and Chiaretti, S. and Foa, R. and Schliwka, J. and Fuchs, U. and Novosel, A. and Mueller, R.U. and Schermer, B. and Bissels, U. and Inman, J. and Phan, Q. and Chien, M. and Weir, D.B. and Choksi, R. and De Vita, G. and Frezzetti, D. and Trompeter, H.I. and Hornung, V. and Teng, G. and Hartmann, G. and Palkovits, M. and Di Lauro, R. and Wernet, P. and Macino, G. and Rogler, C.E. and Nagle, J.W. and Ju, J. and Papavasiliou, F.N. and Benzing, T. and Lichter, P. and Tam, W. and Brownstein, M.J. and Bosio, A. and Borkhardt, A. and Russo, J.J. and Sander, C. and Zavolan, M. and Tuschl, T.
Abstract:	MicroRNAs (miRNAs) are small noncoding regulatory RNAs that reduce stability and/or translation of fully or partially sequence-complementary target mRNAs. In order to identify miRNAs and to assess their expression patterns, we sequenced over 250 small RNA libraries from 26 different organ systems and cell types of human and rodents that were enriched in neuronal as well as normal and malignant hematopoietic cells and tissues. We present expression profiles derived from clone count data and provide computational tools for their analysis. Unexpectedly, a relatively small set of miRNAs, many of which are ubiquitously expressed, account for most of the differences in miRNA profiles between cell lineages and tissues. This broad survey also provides detailed and accurate information about mature sequences, precursors, genome locations, maturation processes, inferred transcriptional units, and conservation patterns. We also propose a subclassification scheme for miRNAs for assisting future experimental and computational functional analyses.
Keywords:	MOLENEURO, MOLIMMUNO, RNA, Animals, Mice, Rats
Source:	Cell
ISSN:	0092-8674
Publisher:	Cell Press
Volume:	129
Number:	7
Page Range:	1401-1414
Date:	29 June 2007
Official Publication:	https://doi.org/10.1016/j.cell.2007.04.040
PubMed:	View item in PubMed

Repository Staff Only: item control page