Molecular characterization of human Argonaute-containing ribonucleoprotein complexes and their bound target mRNAs

Item Type:	Review
Title:	Molecular characterization of human Argonaute-containing ribonucleoprotein complexes and their bound target mRNAs
Creators Name:	Landthaler, M. and Gaidatzis, D. and Rothballer, A. and Chen, P.Y. and Soll, S.J. and Dinic, L. and Ojo, T. and Hafner, M. and Zavolan, M. and Tuschl, T.
Abstract:	microRNAs (miRNAs) regulate the expression of mRNAs in animals and plants through miRNA-containing ribonucleoprotein particles (RNPs). At the core of these miRNA silencing effector complexes are the Argonaute (AGO) proteins that bind miRNAs and mediate target mRNA recognition. We generated HEK293 cell lines stably expressing epitope-tagged human AGO proteins and other RNA silencing-related proteins and used these cells to purify miRNA-containing RNPs. Mass spectrometric analyses of the proteins associated with different AGO proteins revealed a common set of helicases and mRNA-binding proteins, among them the three trinucleotide repeat containing proteins 6 (TNRC6A,-B,-C). mRNA microarray analyses of these miRNA-associated RNPs revealed that AGO and TNRC6 proteins bind highly similar sets of transcripts enriched in binding sites for highly expressed endogenous miRNAs, indicating that the TNRC6 proteins are a component of the mRNA-targeting miRNA silencing complex. Together with the very similar proteomic composition of each AGO complex, this result suggests substantial functional redundancy within families of human AGO and TNRC6 proteins. Our results further demonstrate that we have developed an effective biochemical approach to identify physiologically relevant human miRNA targets.
Keywords:	microRNA, Argonaute, GW182/TNRC6, microRNA Targets, Immunoprecipitation
Source:	RNA
ISSN:	1355-8382
Publisher:	Cold Spring Harbor Laboratory Press
Volume:	14
Number:	12
Page Range:	2580-2596
Date:	December 2008
Official Publication:	https://doi.org/10.1261/rna.1351608
PubMed:	View item in PubMed

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