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DGCR8-dependent microRNA biogenesis is essential for skin development

Item Type:Article
Title:DGCR8-dependent microRNA biogenesis is essential for skin development
Creators Name:Yi, R. and Pasolli, H.A. and Landthaler, M. and Hafner, M. and Ojo, T. and Sheridan, R. and Sander, C. and O'Carroll, D. and Stoffel, M. and Tuschl, T. and Fuchs, E.
Abstract:MicroRNAs play important roles in animal development. Numerous conditional knockout (cKO) studies of Dicer have been performed to interrogate the functions of microRNA during mammalian development. However, because Dicer was recently implicated in the biogenesis of endogenous siRNAs in mammals, it raises the question whether the Dicer cKO defects can be attributable to the loss of microRNAs. Previously, we and others conditionally targeted Dicer and identified its critical roles in embryonic skin morphogenesis. Here, we focus explicitly on microRNAs by taking a parallel strategy with Dgcr8, encoding an essential component of the microprocessor complex that is exclusively required for microRNA biogenesis. With this comparative analysis, we show definitively that the Dicer- and Dgcr8-null skin defects are both striking and indistinguishable. By deep sequencing analysis of microRNA depletion in both Dicer- and Dgcr8-null skin, we demonstrate that most abundantly expressed skin microRNAs are dependent on both Dicer and DGCR8. Our results underscore a specific importance of microRNAs in controlling mammalian skin development.
Keywords:Dicer, Small RNAs, Animals, Mice
Source:Proceedings of the National Academy of Sciences of the United States of America
ISSN:0027-8424
Publisher:National Academy of Sciences (U.S.A.)
Volume:106
Number:2
Page Range:498-502
Date:13 January 2009
Official Publication:https://doi.org/10.1073/pnas.0810766105
PubMed:View item in PubMed

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