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| Item Type: | Article |
|---|---|
| Title: | Formation of cristae and crista junctions in mitochondria depends on antagonism between Fcj1 and Su e/g |
| Creators Name: | Rabl, R. and Soubannier, V. and Scholz, R. and Vogel, F. and Mendl, N. and Vasiljev-Neumeyer, A. and Koerner, C. and Jagasia, R. and Keil, T. and Baumeister, W. and Cyrklaff, M. and Neupert, W. and Reichert, A.S. |
| Abstract: | Crista junctions (CJs) are important for mitochondrial organization and function, but the molecular basis of their formation and architecture is obscure. We have identified and characterized a mitochondrial membrane protein in yeast, Fcj1 (formation of CJ protein 1), which is specifically enriched in CJs. Cells lacking Fcj1 lack CJs, exhibit concentric stacks of inner membrane in the mitochondrial matrix, and show increased levels of F(1)F(O)-ATP synthase (F(1)F(O)) supercomplexes. Overexpression of Fcj1 leads to increased CJ formation, branching of cristae, enlargement of CJ diameter, and reduced levels of F(1)F(O) supercomplexes. Impairment of F(1)F(O) oligomer formation by deletion of its subunits e/g (Su e/g) causes CJ diameter enlargement and reduction of cristae tip numbers and promotes cristae branching. Fcj1 and Su e/g genetically interact. We propose a model in which the antagonism between Fcj1 and Su e/g locally modulates the F(1)F(O) oligomeric state, thereby controlling membrane curvature of cristae to generate CJs and cristae tips. |
| Keywords: | Mitochondrial DNA, Intracellular Membranes, Mitochondria, Mitochondrial Proteins, Mitochondrial Proton-Translocating ATPases, Anatomic Models, Quaternary Protein Structure, Saccharomyces Cerevisiae, Saccharomyces Cerevisiae Proteins |
| Source: | Journal of Cell Biology |
| ISSN: | 0021-9525 |
| Publisher: | Rockefeller University Press |
| Volume: | 185 |
| Number: | 6 |
| Page Range: | 1047-1063 |
| Date: | 15 June 2009 |
| Official Publication: | https://doi.org/10.1083/jcb.200811099 |
| PubMed: | View item in PubMed |
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