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Reexamining microRNA site accessibility in Drosophila: a population genomics study

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Item Type:Article
Title:Reexamining microRNA site accessibility in Drosophila: a population genomics study
Creators Name:Chen, K. and Maaskola, J. and Siegal, M.L. and Rajewsky, N.
Abstract:Kertesz et al. (Nature Genetics 2008) described PITA, a miRNA target prediction algorithm based on hybridization energy and site accessibility. In this note, we used a population genomics approach to reexamine their data and found that the PITA algorithm had lower specificity than methods based on evolutionary conservation at comparable levels of sensitivity.We also showed that deeply conserved miRNAs tend to have stronger hybridization energies to their targets than do other miRNAs. Although PITA had higher specificity in predicting targets than a naïve seed-match method, this signal was primarily due to the use of a single cutoff score for all miRNAs and to the observed correlation between conservation and hybridization energy. Overall, our results clarify the accuracy of different miRNA target prediction algorithms in Drosophila and the role of site accessibility in miRNA target prediction.
Keywords:Amino Acid Substitution, Base Sequence, Population Genetics, Genomics, MicroRNAs, Animals, Drosophila
Source:PLoS ONE
Publisher:Public Library of Science
Page Range:e5681
Date:25 May 2009
Official Publication:https://doi.org/10.1371/journal.pone.0005681
PubMed:View item in PubMed

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