Human ovarian tissue vitrification versus conventional freezing: morphological, endocrinological, and molecular biological evaluation

Item Type:	Article
Title:	Human ovarian tissue vitrification versus conventional freezing: morphological, endocrinological, and molecular biological evaluation
Creators Name:	Isachenko, V. and Lapidus, I. and Isachenko, E. and Krivokharchenko, A. and Kreienberg, R. and Woriedh, M. and Bader, M. and Weiss, J.
Abstract:	Cryopreservation as a process can be divided into two methods: conventional freezing and vitrification. The aim of this study was to compare the safety and effectiveness of conventional freezing and vitrification of human ovarian tissue. Ovarian tissue fragments from 15 patients were transported to the laboratory within 22 to 25 hours in a special, isolated transport box, which can maintain a stable temperature of between 5 degrees and 8 degrees C for 36 hours. Small pieces of ovarian tissue (1 x 1 to 1.5 x 0.7 to 1 mm) were randomly distributed into three groups: Group 1: fresh pieces immediately after receiving transport box (control), Groups 2: pieces after vitrification, and Group 3: pieces after conventional freezing. After thawing all pieces were cultured in vitro. The viability and proliferative capacity of the tissue by in vitro production of hormones, development of follicles and GAPDH-gene expression after culture was evaluated. A difference between freezing and vitrification was not found in respect of hormonal activity and follicle quality. The supernatants showed estradiol 17-beta concentrations of 365, 285, and 300 pg/ml, respectively, and progesterone concentrations of 3.82, 1.99, and 1.95 ng/ml, respectively. It was detected that 95%, 80%, and 83% follicles, respectively, were morphologically normal. The molecular biological analysis, however, has demonstrated that the GAPDH-gene expression in ovarian tissue after vitrification was dramatically decreased in contrast to conventional freezing. For cryopreservation of human ovarian tissue, conventional freezing is more promising than vitrification, because of higher developmental potential.
Keywords:	Analysis of Variance, Apoptosis, Cell Survival, Cryopreservation, Cryoprotective Agents, Culture Media, Estradiol, FreezingGene Expression, Glyceraldehyde-3-Phosphate Dehydrogenases, Organ Culture Techniques, Ovarian Follicle, Ovary, Progesterone, Time Factors
Source:	Reproduction
ISSN:	1470-1626
Publisher:	Bio Scientifica
Volume:	138
Number:	2
Page Range:	319-327
Date:	August 2009
Official Publication:	https://doi.org/10.1530/REP-09-0039
PubMed:	View item in PubMed

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