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Molecular evolution of a novel hyperactive Sleeping Beauty transposase enables robust stable gene transfer in vertebrates

Official URL:https://doi.org/10.1038/ng.343
PubMed:View item in PubMed
Creators Name:Mates, L. and Chuah, M.K. and Belay, E. and Jerchow, B. and Manoj, N. and Acosta-Sanchez, A. and Grzela, D.P. and Schmitt, A. and Becker, K. and Matrai, J. and Ma, L. and Samara-Kuko, E. and Gysemans, C. and Pryputniewicz, D. and Miskey, C. and Fletcher, B. and Vandendriessche, T. and Ivics, Z. and Izsvak, Z.
Journal Title:Nature Genetics
Journal Abbreviation:Nat Genet
Volume:41
Number:6
Page Range:753-761
Date:June 2009
Keywords:Amino Acid Sequence, Conserved Sequence, DNA Transposable Elements, Molecular Evolution, Molecular Sequence Data, Phylogeny, Sequence Alignment, Amino Acid Sequence Homology, Transposases, Vertebrates, Animals, Mice
Abstract:The Sleeping Beauty (SB) transposon is a promising technology platform for gene transfer in vertebrates; however, its efficiency of gene insertion can be a bottleneck in primary cell types. A large-scale genetic screen in mammalian cells yielded a hyperactive transposase (SB100X) with approximately 100-fold enhancement in efficiency when compared to the first-generation transposase. SB100X supported 35-50% stable gene transfer in human CD34(+) cells enriched in hematopoietic stem or progenitor cells. Transplantation of gene-marked CD34(+) cells in immunodeficient mice resulted in long-term engraftment and hematopoietic reconstitution. In addition, SB100X supported sustained (>1 year) expression of physiological levels of factor IX upon transposition in the mouse liver in vivo. Finally, SB100X reproducibly resulted in 45% stable transgenesis frequencies by pronuclear microinjection into mouse zygotes. The newly developed transposase yields unprecedented stable gene transfer efficiencies following nonviral gene delivery that compare favorably to stable transduction efficiencies with integrating viral vectors and is expected to facilitate widespread applications in functional genomics and gene therapy.
ISSN:1061-4036
Publisher:Nature Publishing Group (U.S.A.)
Item Type:Article

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