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Adipocyte plasma membranes contain two Gi subtypes but are devoid of Go

Item Type:Article
Title:Adipocyte plasma membranes contain two Gi subtypes but are devoid of Go
Creators Name:Hinsch, K.D. and Rosenthal, W. and Spicher, K. and Binder, T. and Gausepohl, H. and Frank, R. and Schultz, G. and Joost, H.G.
Abstract:Antisera generated against synthetic peptides were used to identify G-protein alpha-subunits in plasma membranes from rat adipocytes. Applying the immunoblot technique, we detected two Gs alpha-subunits of 42 and 43 kDa, corresponding to the two cholera toxin substrates, and two Gi alpha-subunits of 40 and 41 kDa, corresponding to the two pertussis toxin substrates present in these membranes. The 40 kDa protein was tentatively identified as the Gi2 alpha-subunit. A serum specific for the Go alpha-subunit failed to detect any immunoreactive protein. Thus plasma membranes of adipocytes possess two forms of Gi but not Go.
Keywords:Signal Transduction, G-Protein, Synthetic Peptide, Immunoblotting, Animals, Mice, Rats
Source:FEBS Letters
ISSN:0014-5793
Publisher:Elsevier (The Netherlands)
Volume:238
Number:1
Page Range:191-196
Date:26 September 1988
Official Publication:https://doi.org/10.1016/0014-5793(88)80254-0
PubMed:View item in PubMed

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