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| Item Type: | Article |
|---|---|
| Title: | Different types of ADP-ribose protein bonds formed by botulinum C2 toxin, botulinum ADP-ribosyltransferase C3 and pertussis toxin |
| Creators Name: | Aktories, K. and Just, I. and Rosenthal, W. |
| Abstract: | We attempted to characterize ADP-ribose-amino acid bonds formed by various bacterial toxins. The ADP-ribose-arginine bond formed by botulinum C2 toxin in actin was cleaved with a half-life of about 2 h by treatment with hydroxylamine (0.5 M). In contrast, the ADP-ribose-cysteine bond formed by pertussis toxin in transducin and the ADP-ribose-amino acid linkage formed by botulinum ADP-ribosyltransferase C3 in platelet cytosolic proteins were not affected by hydroxylamine. HgCl2 cleaved the ADP-ribose-amino acid bond formed by pertussis toxin in transducin but not those formed by botulinum C2 toxin or botulinum ADP-ribosyltransferase C3 in actin and platelet cytosolic proteins, respectively. NaOH (0.5 M) cleaved the ADP-ribose-amino acid bonds formed by botulinum C2 toxin and pertussis toxin but not the one formed by botulinum ADP-ribosyltransferase C3. The data indicate that the ADP-ribose bond formed by botulinum ADP-ribosyltransferase C3 differs from those formed by the known bacterial ADP-ribosylating toxins. |
| Keywords: | ADP Ribose Transferases, Adenosine Diphosphate Ribose, Blood Platelets, Blood Proteins, Botulinum Toxins, Cell Membrane, Cytosol, Ethylmaleimide, GTP-Binding Proteins, NAD, Pertussis Toxin, Bordetella Virulence Factors |
| Source: | Biochemical and Biophysical Research Communications |
| ISSN: | 0006-291X |
| Publisher: | Academic Press |
| Volume: | 156 |
| Number: | 1 |
| Page Range: | 361-367 |
| Date: | 14 October 1988 |
| Official Publication: | https://doi.org/10.1016/S0006-291X(88)80849-0 |
| PubMed: | View item in PubMed |
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