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Immunochemical detection of the alpha-subunit of the G-protein, GZ, in membranes and cytosols of mammalian cells

Item Type:Article
Title:Immunochemical detection of the alpha-subunit of the G-protein, GZ, in membranes and cytosols of mammalian cells
Creators Name:Spicher, K. and Hinsch, K.D. and Gausepohl, H. and Frank, R. and Rosenthal, W. and Schultz, G.
Abstract:An antiserum (AS 98) was raised against a synthetic peptide deduced from published cDNA sequences of the alpha-subunit of the putative G-protein, GZ (Fong et al. Proc. Natl. Acad. Sci. USA 85, 3066-3070, 1988; Matsuoka et al. Proc. Natl. Acad. Sci. USA 85, 5384-5388, 1988). In membrane and cytosol preparations of many but not all tested mammalian tissues, AS 98 predominantly recognized two proteins of 40 and 43 kDa Mr. Whereas high levels of a 40 kDa GZ alpha-subunit were found in rat liver membranes and in brain cytosol, AS 98 failed to detect the alpha-subunit of GZ in brain membranes.
Keywords:Amino Acid Sequence, Antibody Specificity, Brain Chemistry, Cell Line, Cell Membrane, Cytosol, Polyacrylamide Gel Electrophoresis, Erythrocytes, GTP-Binding Proteins, Immune Sera, Immunoblotting, Immunohistochemistry, Kidney, Liver, Molecular Sequence Data, Cultured Tumor Cells, Animals, Mice, Rats, Swine
Source:Biochemical and Biophysical Research Communications
ISSN:0006-291X
Publisher:Academic Press (U.S.A.)
Volume:157
Number:3
Page Range:883-890
Date:30 December 1988
Official Publication:https://doi.org/10.1016/S0006-291X(88)80957-4
PubMed:View item in PubMed

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