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The cytosolic G-protein alpha-subunit in human neutrophils responds to treatment with guanine nucleotides and magnesium

Item Type:Article
Title:The cytosolic G-protein alpha-subunit in human neutrophils responds to treatment with guanine nucleotides and magnesium
Creators Name:Rudolph, U. and Schultz, G. and Rosenthal, W.
Abstract:The hydrodynamic properties of the Gi2 alpha-subunit in human neutrophil cytosol were analyzed. The S20,w value was 3.3 S at 30 degrees C and 4.1 S at 4 degrees C. Reconstitution of the cytosolic alpha-subunit with beta gamma-complex purified from porcine brain resulted in an S20,w value of 4.0 S at 30 degrees C and 4.2 S at 4 degrees C. Treatment of cytosol with G-protein-activating agents, GTP gamma S and MgCl2, decreased the S20,w value to 2.4 S at 30 degrees C and 2.9 S at 4 degrees C. Our results indicate that the cytosolic alpha-subunit of neutrophils is an inactive monomeric species at 30 degrees C capable of interacting with G-protein beta gamma-complex and responsive to treatment with activating agents leading to the two activated forms, alpha at 4 degrees C and alpha at 30 degrees C.
Keywords:Signal Transduction, Guanine Nucleotide Binding Regulatory Protein, Guanine Nucleotide, Neutrophil, Cytosol, Animals, Swine
Source:FEBS Letters
ISSN:0014-5793
Publisher:Elsevier (The Netherlands)
Volume:251
Number:1-2
Page Range:137-142
Date:17 July 1989
Official Publication:https://doi.org/10.1016/0014-5793(89)81443-7
PubMed:View item in PubMed

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