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Molecular cloning of the receptor for human antidiuretic hormone

Item Type:Article
Title:Molecular cloning of the receptor for human antidiuretic hormone
Creators Name:Birnbaumer, M. and Seibold, A. and Gilbert, S. and Ishido, M. and Barberis, C. and Antaramian, A. and Brabet, P. and Rosenthal, W.
Abstract:Antidiuresis, the recovery of water from the lumen of the renal collecting tubule, is regulated by the hypothalamic release of antidiuretic hormone (ADH), which binds to specific receptors on renal collecting tubule cells, stimulates adenylyl cyclase and promotes the cyclic AMP-mediated incorporation of water pores into the luminal surface of these cells. We report here the isolation of the human ADH receptor gene using a genomic expression cloning approach. The gene was used to clone the complementary DNA from a human renal library. The deduced amino-acid sequence of the receptor yields a hydropathy profile characteristic of receptors with seven putative transmembrane regions. This and the comparison with other cloned receptors indicates that the ADH receptor is a member of the superfamily of G-protein-coupled receptors.
Keywords:Amino Acid Sequence, Arginine Vasopressin, Bacteriophages, Base Sequence, Southern Blotting, Molecular Cloning, Cosmids, DNA, Molecular Sequence Data, Angiotensin Receptors, Vasopressin Receptors, Nucleic Acid Repetitive Sequences, Restriction Mapping, Transfection
Source:Nature
ISSN:0028-0836
Publisher:Nature Publishing Group (U.K.)
Volume:357
Number:6376
Page Range:333-335
Date:28 May 1992
Official Publication:https://doi.org/10.1038/357333a0
PubMed:View item in PubMed

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