A heterotrimeric G protein of the Gi family is required for cAMP-triggered trafficking of aquaporin 2 in kidney epithelial cells

Item Type:	Article
Title:	A heterotrimeric G protein of the Gi family is required for cAMP-triggered trafficking of aquaporin 2 in kidney epithelial cells
Creators Name:	Valenti, G. and Procino, G. and Liebenhoff, U. and Frigeri, A. and Benedetti, P.A. and Ahnert-Hilger, G. and Nuernberg, B. and Svelto, M. and Rosenthal, W.
Abstract:	Vasopressin is the key regulator of water homeostasis in vertebrates. Central to its antidiuretic action in mammals is the redistribution of the water channel aquaporin 2 (AQP2) from intracellular vesicles to the apical membrane of kidney epithelial cells, an event initiated by an increase in cAMP and activation of protein kinase A. The subsequent steps of the signaling cascade are not known. To identify proteins involved in the AQP2 shuttle we exploited a recently developed cell line (CD8) derived from the rabbit cortical collecting duct and stably transfected with rat AQP2 cDNA. Treatment of CD8 cells with pertussis toxin (PTX) inhibited both the vasopressin-induced increase in water permeability and the redistribution of AQP2 from an intracellular compartment to the apical membrane. ADP-ribosylation studies revealed the presence of at least two major PTX substrates. Correspondingly, two alpha subunits of PTX-sensitive G proteins, Galphai2 and Galphai3, were identified by Western blotting. Introduction of a synthetic peptide corresponding to the C terminus of the Gi3 alpha subunit into permeabilized CD8 cells efficiently inhibited the cAMP-induced AQP2 translocation; a peptide corresponding to the alpha subunits of Gi1/2 was much less potent. Thus a member of the Gi family, most likely Gi3, is involved in the cAMP-triggered targeting of AQP2-bearing vesicles to the apical membrane of kidney epithelial cells.
Keywords:	Amino Acid Sequence, Aquaporin 2, Aquaporin 6, Aquaporins, Biological Transport, Cell Line, Cyclic AMP, Complementary DNA, Epithelial Cells, Forskolin, GTP-Binding Proteins, Ion Channels, Collecting Kidney Tubules, Confocal Microscopy, Fluorescence Microscopy, Pertussis Toxin, Bordetella Virulence Factors, Animals, Rats, Rabbits
Source:	Journal of Biological Chemistry
ISSN:	0021-9258
Publisher:	American Society for Biochemistry and Molecular Biology
Volume:	273
Number:	35
Page Range:	22627-22634
Date:	28 August 1998
Official Publication:	http://www.jbc.org/cgi/content/abstract/273/35/22627
PubMed:	View item in PubMed

Repository Staff Only: item control page