Vasopressin-induced von Willebrand factor secretion from endothelial cells involves V2 receptors and cAMP

Item Type:	Article
Title:	Vasopressin-induced von Willebrand factor secretion from endothelial cells involves V2 receptors and cAMP
Creators Name:	Kaufmann, J.E. and Oksche, A. and Wollheim, C.B. and Guenther, G. and Rosenthal, W. and Vischer, U.M.
Abstract:	Vasopressin and its analogue 1-deamino-8-D-arginine vasopressin (DDAVP) are known to raise plasma von Willebrand factor (vWF) levels. DDAVP is used as a hemostatic agent for the treatment of von Willebrand's disease. However, its cellular mechanisms of action have not been elucidated. DDAVP, a specific agonist for the vasopressin V2 receptor (V2R), exerts its antidiuretic effect via a rise in cAMP in kidney collecting ducts. We tested the hypothesis that DDAVP induces vWF secretion by binding to V2R and activating cAMP-mediated signaling in endothelial cells. vWF secretion from human umbilical vein endothelial cells (HUVECs) can be mediated by cAMP, but DDAVP is ineffective, presumably due to the absence of V2R. We report that DDAVP stimulates vWF secretion in a cAMP-dependent manner in HUVECs after transfection of the V2R. In addition, vasopressin and DDAVP induce vWF secretion in human lung microvascular endothelial cells (HMVEC-L). These cells (but not HUVECs) express endogenous V2R, as shown by RT-PCR. Vasopressin-induced vWF secretion is mimicked by DDAVP and inhibited by the selective V2R antagonist SR121463B. It is mediated by cAMP, since it is inhibited by the protein kinase A inhibitor Rp-8CPT-cAMPS. These results indicate that vasopressin induces cAMP-mediated vWF secretion by a direct effect on endothelial cells. They also demonstrate functional expression of V2R in endothelial cells, and provide a cellular mechanism for the hemostatic effects of DDAVP.
Keywords:	Arginine Vasopressin, Cultured Cells, Cyclic AMP, Deamino Arginine Vasopressin, Vascular Endothelium, Lung, Messenger RNA, Vasopressin Receptors, Reverse Transcriptase Polymerase Chain Reaction, von Willebrand Factor
Source:	Journal of Clinical Investigation
ISSN:	0021-9738
Publisher:	American Society for Clinical Investigation
Volume:	106
Number:	1
Page Range:	107-116
Date:	July 2000
Official Publication:	https://doi.org/10.1172/JCI9516
PubMed:	View item in PubMed

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