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Ht31: the first protein kinase A anchoring protein to integrate protein kinase A and Rho signaling

Item Type:Article
Title:Ht31: the first protein kinase A anchoring protein to integrate protein kinase A and Rho signaling
Creators Name:Klussmann, E. and Edemir, B. and Pepperle, B. and Tamma, G. and Henn, V. and Klauschenz, E. and Hundsrucker, C. and Maric, K. and Rosenthal, W.
Abstract:In an attempt to isolate protein kinase A anchoring proteins (AKAPs) involved in vasopressin-mediated water reabsorbtion, the complete sequence of the human AKAP Ht31 was determined and a partial cDNA of its rat orthologue (Rt31) was cloned. The Ht31 cDNA includes the estrogen receptor cofactor Brx and the RhoA GDP/GTP exchange factor proto-lymphoid blast crisis (Lbc) sequences. The Ht31 gene was assigned to chromosome 15 (region q24-q25). It encodes Ht31 and the smaller splice variants Brx and proto-Lbc. A protein of the predicted size of Ht31 (309 kDa) was detected in human mammary carcinoma and HeLa cells. Anti-Ht31/Rt31 antibodies immunoprecipitated RhoA from primary cultured rat renal inner medullary collecting duct cells, indicating an interaction between the AKAP and RhoA in vivo. These results suggest that Ht31/Rt31 represent a new type of AKAP, containing both an anchoring and a catalytic domain, which appears to be capable of modulating the activity of an interacting partner. Ht31/Rt31 have the potential to integrate Rho and protein kinase A signaling pathways, and thus, are prime candidates to regulate vasopressin-mediated water reabsorbtion.
Keywords:Protein kinase A anchoring protein, Rho, Rho GTPase guanine nucleotide exchange factor, Ht31, Brx, Aquaporin, Animals, Rats
Source:FEBS Letters
Page Range:264-268
Date:2 November 2001
Official Publication:https://doi.org/10.1016/S0014-5793(01)02995-7
PubMed:View item in PubMed

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