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Actin remodeling requires ERM function to facilitate AQP2 apical targeting

Item Type:Article
Title:Actin remodeling requires ERM function to facilitate AQP2 apical targeting
Creators Name:Tamma, G. and Klussmann, E. and Oehlke, J. and Krause, E. and Rosenthal, W. and Svelto, M. and Valenti, G.
Abstract:This study provides the first evidence that actin reorganization during AQP2 vesicular trafficking to the plasma membrane requires the functional involvement of ERM (ezrin/radixin/moesin) proteins cross-linking actin filaments with plasma membrane proteins. We report that forskolin stimulation was associated with a redistribution of moesin from intracellular sites to the cell cortex and with a concomitant enrichment of moesin in the particulate fraction in renal cells. Introduction of a peptide reproducing a short sequence of moesin within the binding site for F-actin induced all the key effects of forskolin stimulation, including a decrease in F-actin, translocation of endogenous moesin, and AQP2 translocation. A straightforward explanation for these effects is the ability of the peptide to uncouple moesin from its putative effector. This modifies the balance between the active and inactive forms of moesin. Extraction with Triton X-100, which preserves cytoskeletal associated proteins, showed that forskolin stimulation or peptide introduction reduced the amount of phophorylated moesin, a molecular modification known to stabilize moesin in an active state. Our data point to a dual role of moesin in AQP2 trafficking: it might modulate actin depolymerization and it participates in the reorganization of F-actin-containing cytoskeletal structures close to the fusion sites of the AQP2-bearing vesicles.
Keywords:Aquaporin, ERM, Actin Cytoskeleton, Animals, Rabbits, Rats
Source:Journal of Cell Science
Publisher:Company of Biologists
Number:Pt 16
Page Range:3623-3630
Date:15 August 2005
Additional Information:Erratum in: J Cell Sci 134(22): jcs259467
Official Publication:https://doi.org/10.1242/jcs.02495
PubMed:View item in PubMed

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