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Expression, purification and preliminary X-ray crystallographic analysis of the chicken MHC class I molecule YF1*7.1

Item Type:Article
Title:Expression, purification and preliminary X-ray crystallographic analysis of the chicken MHC class I molecule YF1*7.1
Creators Name:Hee, C.S. and Gao, S. and Miller, M.M. and Goto, R.M. and Ziegler, A. and Daumke, O. and Uchanska-Ziegler, B.
Abstract:YF1*7.1 is an allele of a polymorphic major histocompatibility complex (MHC) class I-like locus within the chicken Y gene complex. With the aim of understanding the possible role of the YF1*7.1 molecule in antigen presentation, the complex of YF1*7.1 heavy chain and beta(2)-microglobulin was reconstituted and purified without a peptide. Crystals diffracted synchrotron radiation to 1.32 A resolution and belonged to the monoclinic space group P2(1). The phase problem was solved by molecular replacement. A detailed examination of the structure may provide insight into the type of ligand that could be bound by the YF1*7.1 molecule.
Keywords:YF1*7.1, Chicken Rfp-Y, MHC Class I Antigens, X-Ray Crystallography, Histocompatibility Antigens Class I, beta 2-Microglobulin, Animals, Chickens
Source:Acta Crystallographica Section F
ISSN:1744-3091
Publisher:International Union of Crystallography (U.K.)
Volume:65
Number:Pt 4
Page Range:422-425
Date:1 April 2009
Additional Information:Erratum in: Acta Crystallographica Section F 66(Pt.2): 214
Official Publication:https://doi.org/10.1107/S1744309109009026
PubMed:View item in PubMed

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