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| Item Type: | Article |
|---|---|
| Title: | Aberrant methylation of human L- and M-fructose 1,6-bisphosphatase genes in cancer |
| Creators Name: | Bigl, M., Jandrig, B., Horn, L.C. and Eschrich, K. |
| Abstract: | A possible epigenetic regulation of the two isoenzymes of fructose 1,6-bisphosphatase (FBPase) was studied in liver, muscle, mamma, breast cancer and in different cancer cell lines. Results obtained after bisulfite sequencing revealed a different CpG methylation of both promoters in liver, muscle and breast tissue which is putatively involved in the cell-type specific gene expression of the two enzymes. In tumor cell lines, demethylation with 5-aza-deoxycytidine activated the expression of both isoenzymes. Additional inhibition of histone deacetylase with trichostatin A further increased FBPase mRNA concentrations. Since cancers typically have an abnormal energy metabolism and exhibit a low gluconeogenic phenotype, it was studied whether promoter methylation contributes to the decreased expression of FBPase in breast cancer. When non-malignant and malignant tissue samples from the same patient were compared a correlation between an increase of FBPase promoter methylation and a decrease of FBPase mRNA levels was observed. |
| Keywords: | Breast Cancer, Cell Line, DNA Methylation, Fructose 1,6-Bisphosphatase, Gene Expression, Gluconeogenesis, Promoter, Tissue-Specific Expression |
| Source: | Biochemical and Biophysical Research Communications |
| ISSN: | 0006-291X |
| Publisher: | Academic Press |
| Volume: | 377 |
| Number: | 2 |
| Page Range: | 720-724 |
| Date: | 12 December 2008 |
| Official Publication: | https://doi.org/10.1016/j.bbrc.2008.10.045 |
| PubMed: | View item in PubMed |
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