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| Item Type: | Article |
|---|---|
| Title: | Temperature and nitric oxide control spontaneous calcium transients in astrocytes |
| Creators: |
Schipke, C.G.  ORCID: https://orcid.org/0000-0002-1105-1853, Heidemann, A., Skupin, A. ORCID: https://orcid.org/0000-0002-8955-8304, Peters, O. ORCID: https://orcid.org/0000-0003-0568-2998, Falcke, M. ORCID: https://orcid.org/0000-0001-7137-1114 and Kettenmann, H. ORCID: https://orcid.org/0000-0001-8208-0291
|
| Abstract: | Transient spontaneous increases in the intracellular Ca2+ concentration have been frequently observed in astrocytes in cell culture and in acutely isolated slices from several brain regions. Recent in vivo experiments, however, reported only a low frequency of spontaneous Ca2+ events in astrocytes. Since the ex vivo experiments were usually performed at temperatures lower than physiological body temperature, we addressed the question whether temperature could influence the spontaneous Ca2+ activity in astrocytes. Indeed, comparing the frequency and spike width of spontaneous Ca2+ transients in astrocytes at temperatures between 20 and 37 °C in culture as well as in acute cortical slices from mouse brain, revealed that spontaneous Ca2+ responses occurred frequently at low temperature and became less frequent at higher temperature. Moreover, the single Ca2+ events had a longer duration at low temperature. We found that nitric oxide (NO) mimicked the increase in spontaneous Ca2+ activity and that an NO-synthase inhibitor attenuated the effect of lowering the temperature. Thus, temperature and NO are major determinants of spontaneous astrocytic Ca2+ signalling. |
| Keywords: | Calcium, Astrocyte, Glia, Nitric oxide, Brain slice, Cell culture, Temperature, Animals, Mice |
| Source: | Cell Calcium |
| ISSN: | 0143-4160 |
| Publisher: | Elsevier |
| Volume: | 43 |
| Number: | 3 |
| Page Range: | 285-295 |
| Date: | March 2008 |
| Additional Information: | The original publication is available at www.sciencedirect.com |
| Official Publication: | https://doi.org/10.1016/j.ceca.2007.06.002 |
| PubMed: | View item in PubMed |
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