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| Item Type: | Article |
|---|---|
| Title: | MeCP2 interacts with HP1 and modulates its heterochromatin association during myogenic differentiation |
| Creators Name: | Agarwal, N., Hardt, T., Brero, A., Nowak, D., Rothbauer, U., Becker, A., Leonhardt, H. and Cardoso, M.C. |
| Abstract: | There is increasing evidence of crosstalk between epigenetic modifications such as histone and DNA methylation, recognized by HP1 and methyl CpG-binding proteins, respectively. We have previously shown that the level of methyl CpG-binding proteins increased dramatically during myogenesis leading to large-scale heterochromatin reorganization. In this work, we show that the level of HP1 isoforms did not change significantly throughout myogenic differentiation but their localization did. In particular, HP1 relocalization to heterochromatin correlated with MeCP2 presence. Using co-immunoprecipitation assays, we found that these heterochromatic factors interact in vivo via the chromo shadow domain of HP1 and the first 55 amino acids of MeCP2. We propose that this dynamic interaction of HP1 and MeCP2 increases their concentration at heterochromatin linking two major gene silencing pathways to stabilize transcriptional repression during differentiation. |
| Keywords: | Binding Sites, Cell Differentiation, Cell Line, Non-Histone Chromosomal Proteins, Heterochromatin, Methyl-CpG-Binding Protein 2, Muscle Development, Myoblasts, Protein Isoforms, Tertiary Protein Structure, Animals, Mice, Rats |
| Source: | Nucleic Acids Research |
| ISSN: | 0305-1048 |
| Publisher: | Oxford University Press |
| Volume: | 35 |
| Number: | 16 |
| Page Range: | 5402-5408 |
| Date: | August 2007 |
| Official Publication: | https://doi.org/10.1093/nar/gkm599 |
| PubMed: | View item in PubMed |
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