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| Item Type: | Article |
|---|---|
| Title: | Stable chromosomal units determine the spatial and temporal organization of DNA replication |
| Creators Name: | Sadoni, N., Cardoso, M.C., Stelzer, E.H., Leonhardt, H. and Zink, D. |
| Abstract: | DNA replication occurs in mammalian cells at so-called replication foci occupying defined nuclear sites at specific times during S phase. It is an unresolved problem how this specific spatiotemporal organization of replication foci is determined. Another unresolved question remains as to what extent DNA is redistributed during S phase. To investigate these problems, we visualized the replicating DNA and the replication machinery simultaneously in living HeLa cells. Time-lapse analyses revealed that DNA was not redistributed to other nuclear sites during S phase. Furthermore, the results showed that DNA is organized into stable aggregates equivalent to replication foci. These aggregates, which we call sub-chromosomal foci, stably maintained their replication timing from S phase to S phase. During S-phase progression, the replication machinery sequentially proceeded through spatially adjacent sets of sub-chromosomal foci. These findings imply that the specific nuclear substructure of chromosomes and the order of their stable subunits determine the spatiotemporal organization of DNA replication. |
| Keywords: | Replication Dynamics, Replication Foci, Sub-Chromosomal Foci, Animals, Drosophila |
| Source: | Journal of Cell Science |
| ISSN: | 0021-9533 |
| Publisher: | Company of Biologists |
| Volume: | 117 |
| Number: | 22 |
| Page Range: | 5353-5365 |
| Date: | 15 October 2004 |
| Official Publication: | https://doi.org/10.1242/jcs.01412 |
| PubMed: | View item in PubMed |
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