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| Item Type: | Article |
|---|---|
| Title: | Properties of GABA and glutamate responses in identified glial cells of mouse hippocampal slice |
| Creators Name: | Steinhaeuser, C., Jabs, R. and Kettenmann, H. |
| Abstract: | In this study, the patch-clamp technique was applied to brain slices to test for the presence of GABAA and glutamate receptors in glial cells of an intact tissue preparation, the hippocampus from 9-12 day old mice. Two types of glial cells were studied in the CA1 stratum pyramidale, termed passive and complex cells, which were distinct by their characteristic pattern of voltage-dependent currents. Both cell types were previously identified as glial by combining electrophysiology with ultrastructural inspection (Steinhüser et al., 1992, Eur J Neurosci 4:472-484). A subpopulation of passive cells was positive, all complex cells were negative for immunocytochemical staining against glial fibrillary acidic protein, a marker of mature astrocytes. In both cell types, GABA activated currents compatible with GABAA-receptor mediated responses. The glutamate response in complex and in most of the passive cells was mediated by a ligand-gated ion channel and closely matched the pharmacology of the kainate receptor. Activation of glutamate receptors led to a transient decrease of the resting K+ conductance in complex cells and to an irreversible decrease in the passive cells. In three passive cells, glutamate-activated currents were most likely dominated by an electrogenic uptake. In a small group of passive cells NMDA-activated currents were observed. This study provides evidence that glial cells from an intact tissue express receptors for the most abundant transmitters in the central nervous system, glutamate, and GABA. |
| Keywords: | Barium, Electrodes, Electrophysiology, Glial Fibrillary Acidic Protein, Glutamates, Glutamic Acid, Hippocampus, Immunohistochemistry, Kainic Acid, N-Methylaspartate, Neuroglia, Potassium Channels, GABA-A Receptors, Glutamate Receptors |
| Source: | Hippocampus |
| ISSN: | 1050-9631 |
| Publisher: | Wiley |
| Volume: | 4 |
| Number: | 1 |
| Page Range: | 19-36 |
| Date: | 1 February 1994 |
| Official Publication: | https://doi.org/10.1002/hipo.450040105 |
| PubMed: | View item in PubMed |
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