High pressure as tool to study structural properties of thiolate heme proteins

Tools

Item Type:	Book Section
Title:	High pressure as tool to study structural properties of thiolate heme proteins
Creators Name:	Jung, C., Canny, B. and Chervin, J.C.
Abstract:	A membrane driven high-pressure cell with sapphire anvils is used to measure the CO ligand stretch mode of various substrate complexes of cytochrome P450cam. In situ pressure measurement is performed using the ruby R1 luminescence band using a Raman spectrometer coupled via fiber optics to the sample compartment of the FT infrared spectrometer. It was found for all substrate complexes that the CO stretch mode shifts to lower frequencies with increasing pressure. The slope of the shift is interpretated as compressibility and correlates with the high-spin state content produced by the substrates in the oxidized form of the enzyme.
Title of Book:	Advances in High-Pressure Biosciences and Biotechnology
ISBN:	978-3-642-05674-1
Publisher:	Springer
Page Range:	19-24
Date:	2003
Official Publication:	https://doi.org/10.1007/978-3-662-05613-4_4

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