Sedimentation equilibrium: a valuable tool to study homologous and heterogeneous interactions of proteins or proteins and nucleic acids

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Item Type:	Article
Title:	Sedimentation equilibrium: a valuable tool to study homologous and heterogeneous interactions of proteins or proteins and nucleic acids
Creators Name:	Behlke, J. and Ristau, O.
Abstract:	We present a short overview of our experience in analyzing the affinity and stoichiometry of self-associating and heterologous interactions by using the sedimentation equilibrium technique. Data acquisition and the fitting procedure employing the computer programs that we have developed, Polymole and Virial, are utilized for obtaining reliable results under ideal as well as non-ideal conditions. Such data derived from biologically important macromolecules find utility in understanding physiological events such as cell regulation.
Keywords:	Analytical Ultracentrifugation, Binding Constants, Heterogeneous Association, Self-Association, Virial Coefficients
Source:	European Biophysics Journal with Biophysics Letters
ISSN:	0175-7571
Publisher:	Springer
Volume:	32
Number:	5
Page Range:	427-431
Date:	1 August 2003
Official Publication:	https://doi.org/10.1007/s00249-003-0318-7
PubMed:	View item in PubMed

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