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| Item Type: | Article |
|---|---|
| Title: | Charting the proteomes of organisms with unsequenced genomes by MALDI-quadrupole time-of-flight mass spectrometry and BLAST homology searching |
| Creators Name: | Shevchenko, A., Sunyaev, S., Loboda, A., Shevchenko, A., Bork, P., Ens, W. and Standing, K.G. |
| Abstract: | MALDI-quadrupole time-of-flight mass spectrometry was applied to identify proteins from organisms whose genomes are still unknown. The identification was carried out by successively searching a sequence database-first with a peptide mass fingerprint, then with a packet of noninterpreted MS/MS spectra, and finally with peptide sequences obtained by automated interpretation of the MS/MS spectra. A "MS BLAST" homology searching protocol was developed to overcome specific limitations imposed by mass spectrometric data, such as the limited accuracy of de novo sequence predictions. This approach was tested in a small-scale proteomic project involving the identification of 15 bands of gel-separated proteins from the methylotrophic yeast Pichia pastoris, whose genome has not yet been sequenced and which is only distantly related to other fungi. |
| Keywords: | Algorithms, Amino Acid Sequence, Cell Line, Factual Databases, Fungal Genome, Kidney, Membrane Proteins, Molecular Sequence Data, Peptide Mapping, Pichia, Proteome, Protein Sequence Analysis, Mass, Matrix-Assisted Laser Desorption-Ionization Spectrometry, Trypsin, Animals, Dogs |
| Source: | Analytical Chemistry |
| ISSN: | 0003-2700 |
| Publisher: | American Chemical Society |
| Volume: | 73 |
| Number: | 9 |
| Page Range: | 1917-1926 |
| Date: | 1 May 2001 |
| Official Publication: | https://doi.org/10.1021/ac0013709 |
| PubMed: | View item in PubMed |
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