DANA elements: a family of composite, tRNA-derived short interspersed DNA elements associated with mutational activities in zebrafish (Danio rerio)

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Item Type:	Article
Title:	DANA elements: a family of composite, tRNA-derived short interspersed DNA elements associated with mutational activities in zebrafish (Danio rerio)
Creators Name:	Izsvak, Z., Ivics, Z., Garcia-Estefania, D., Fahrenkrug, S.C. and Hackett, P.B.
Abstract:	DNA is the first SINE isolated from zebrafish (Danio rerio) exhibiting all the hallmarks of these tRNA-derived elements. DANA is unique in its clearly defined substructure of distinct cassettes. In contrast to generic SINE elements, DANA appears to have been assembled by insertions of short sequences into a progenitor, tRNA-derived element. Once associated with each other, these subunits were amplified as a new transposable element with such a remarkable success that DANA-related sequences comprise approximately 10% of the modern zebrafish genome. At least some of the sequences comprised by the full-length element were capable of movement, forming a new group of mobile, composite transposons, one of which caused an insertional mutation in the zebrafish no tail gene. Being present only in the genus Danio, and estimated to be as old as the genus itself, DANA may have played a role in Danio speciation by massive amplification and genome-wide dispersion. There are extensive DNA polymorphisms between zebrafish populations and strains detected by PCR amplification using primers specific to DANA, suggesting that the DANA element will be useful as a molecular tool for genetic and phylogenetic analyses.
Keywords:	Base Sequence, Southern Blotting, Molecular Cloning, Conserved Sequence, DNA Primers, DNA Transposable Elements, Molecular Sequence Data, Mutation, Polymerase Chain Reaction, Genetic Polymorphism, Transfer RNA, Nucleic Acid Repetitive Sequences, Retroelements, Nucleic Acid Sequence Homology, Animals, Zebrafish
Source:	Proceedings of the National Academy of Sciences of the United States of America
ISSN:	0027-8424
Publisher:	National Academy of Sciences
Volume:	93
Number:	3
Page Range:	1077-1081
Date:	6 February 1996
Official Publication:	https://doi.org/10.1073/pnas.93.3.1077
PubMed:	View item in PubMed

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