Cloning and initial analysis of the human multidrug resistance-related MVP/LRP gene promoter

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Item Type:	Article
Title:	Cloning and initial analysis of the human multidrug resistance-related MVP/LRP gene promoter
Creators Name:	Lange, C., Walther, W., Schwabe, H. and Stein, U.
Abstract:	The lung resistance-related protein (LRP) was identified as the human major vault protein (MVP), and is overexpressed in various multidrug-resistant cancer cell lines and clinical samples. We characterized DNA sequences upstream to the transcription initiation site of the MVP gene in the human non-small cell lung cancer cell line SW-1573. A 1.9-kb and a shortened 0.7-kb fragment of the 5'-upstream genomic region show strong promoter activity in chloramphenicol acetyltransferase (CAT) reporter assays. The promoter is TATA-less and contains an inverted CCAAT-box and a Sp1 site located near to a p53 binding motif. An alternative 3'-splice site of intron 1 results in a splicing variant within the 5'-untranslated region of MVP mRNA.
Keywords:	Gene Promoter, Human Major Vault Protein/Lung Resistance-Related Protein, Multidrug Resistance
Source:	Biochemical and Biophysical Research Communications
ISSN:	0006-291X
Publisher:	Academic Press
Volume:	278
Number:	1
Page Range:	125-133
Date:	11 November 2000
PubMed:	View item in PubMed

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