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| Item Type: | Article |
|---|---|
| Title: | Allogeneic vaccination for renal cell carcinoma: development and monitoring |
| Creators: |
Pohla, H., Frankenberger, B., Stadlbauer, B., Oberneder, R., Hofstetter, A., Willimsky, G.  ORCID: https://orcid.org/0000-0002-9693-948X, Pezzutto, A. ORCID: https://orcid.org/0000-0002-3246-4470, Doerken, B. ORCID: https://orcid.org/0000-0001-7991-3643, Blankenstein, T. ORCID: https://orcid.org/0000-0002-3357-4321 and Schendel, D.J.
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| Abstract: | An allogeneic tumor cell vaccine should display a natural immunogenicity that allows the stimulation of tumor-reactive effector cells in patients. Furthermore, the vaccine should express antigens that are shared by many tumors to which patients are not tolerant. A variety of tumor peptides should be presented by different HLA-molecules due to limited MHC matching with recipients and last but not least, the vaccine should have a strong growth potential in vitro to allow adequate amounts of vaccine to be generated for long-term usage. In vitro and in situ studies with the renal cell carcinoma cell line RCC-26 demonstrate: (1) RCC-26 can induce complex allospecific responses through direct priming; (2) RCC-26 can not only reactivate cytotoxic T lymphocytes (CTL) of a memory phenotype but they also can induce de novo tumor-antigen associated responses in normal donors; (3) these cells present epitopes restricted by several MHC molecules, allowing the vaccination of patients matched for different HLA alleles; and (4) they stimulate HLA-A*0201-restricted T cells bearing characteristic T cell receptors (TCR). Thus, in addition to using limiting dilution killer and ELISPOT assays, molecular tracking of a tumor-specific TCR can be used to judge the development of antitumor reactivity and vaccine efficiency. |
| Keywords: | Allogeneic Vaccination, Chromium Release, ELISPOT, Immune Monitoring, Lanthanide Release, Renal Cell Carcinoma, TCR Tracking |
| Source: | Bone Marrow Transplantation |
| ISSN: | 0268-3369 |
| Publisher: | Nature Publishing Group |
| Volume: | 25 Suppl. 2 |
| Number: | 3332 |
| Page Range: | S83-S87 |
| Date: | 1 May 2000 |
| Official Publication: | http://www.nature.com/bmt/journal/v25/n2s/abs/1702362a.html |
| PubMed: | View item in PubMed |
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