Nucleolin and YB-1 are required for JNK-mediated interleukin-2 mRNA stabilization during T-cell activation

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Item Type:	Article
Title:	Nucleolin and YB-1 are required for JNK-mediated interleukin-2 mRNA stabilization during T-cell activation
Creators Name:	Chen, C.Y., Gherzi, R., Andersen, J.S., Gaietta, G., Juerchott, K., Royer, H.D., Mann, M. and Karin, M.
Abstract:	Regulated mRNA turnover is a highly important process, but its mechanism is poorly understood. Using interleukin-2 (IL-2) mRNA as a model, we described a role for the JNK-signaling pathway in stabilization of IL-2 mRNA during T-cell activation, acting via a JNK response element (JRE) in the 5' untranslated region (UTR). We have now identified two major RNA-binding proteins, nucleolin and YB-1, that specifically bind to the JRE. Binding of both proteins is required for IL-2 mRNA stabilization induced by T-cell activation signals and for JNK-induced stabilization in a cell-free system that duplicates essential features of regulated mRNA decay. Nucleolin and YB. 1 are required for formation of an IL-2 mRNP complex that responds to specific mRNA stabilizing signals.
Keywords:	JNK, Nucleolin, Stabilization, T-cell Activation, Trans-Acting Factors
Source:	Genes & Development
ISSN:	0890-9369
Publisher:	Cold Spring Harbor Laboratory Press
Volume:	14
Number:	10
Page Range:	1236-1248
Date:	15 May 2000
Official Publication:	https://doi.org/10.1101/gad.14.10.1236
PubMed:	View item in PubMed

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