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| Item Type: | Article |
|---|---|
| Title: | NKX2.3 is required for MAdCAM-1 expression and homing of lymphocytes in spleen and mucosa-associated lymphoid tissue |
| Creators Name: | Pabst, O., Foerster, R., Lipp, M., Engel, H. and Arnold, H.H. |
| Abstract: | Targeted disruption of the transcription factor NKX2.3 gene in mice results in anatomical defects of intestine and secondary lymphoid organs. Here, we report that spleen and Peyer's patches of NKX2.3-deficient mice are considerably reduced in size and lack the ordered tissue architecture. T and B cells are misplaced within the spleen and mesenteric lymph nodes and fail to segregate into the appropriate T and B cell areas. Furthermore, splenic marginal zones, characterized by specific B cells and various types of macrophage-derived cells around the marginal sinus, are absent in mutants. Homozygous NKX2.3 mutants lack the mucosal addressin cell adhesion molecule-1 (MAdCAM-1) that is normally expressed in mucosa-associated lymphoid tissue (MALT) and spleen. We provide evidence that NKX2.3 can activate MAdCAM-1 transcription directly, suggesting that MAdCAM-1 is at least partly responsible for the migration and homing defects of lymphocytes and macrophages in mutants. Therefore, expression of MAdCAM-1 seems to be required for building functional structures in spleen and MALT, a prerequisite for unimpaired migration and segregation of B and T cells to and within these organs. |
| Keywords: | Lymphocyte Homing, MAdCAM-1 Expression, NKX2.3 Knock-Out, Spleen Anatomy, T and B Lymphocytes, Animals, Mice |
| Source: | EMBO Journal |
| ISSN: | 0261-4189 |
| Publisher: | Oxford University Press |
| Volume: | 19 |
| Number: | 9 |
| Page Range: | 2015-2023 |
| Date: | 1 January 2000 |
| Official Publication: | https://doi.org/10.1093/emboj/19.9.2015 |
| PubMed: | View item in PubMed |
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