Helmholtz Gemeinschaft

Search
Browse
Statistics
Feeds

Mammalian mitochondrial ribosomal proteins (2) - Amino acid sequencing, characterization, and identification of corresponding gene sequences

Item Type:Article
Title:Mammalian mitochondrial ribosomal proteins (2) - Amino acid sequencing, characterization, and identification of corresponding gene sequences
Creators Name:O'Brien, T.W., Fiesler, S.E., Denslow, N.D., Thiede, B., Wittmann-Liebold, B., Mougey, E.B., Sylvester, J.E. and Graack, H.R.
Abstract:Four different classes of mammalian mitochondrial ribosomal proteins were identified and characterized. Mature proteins were purified from bovine liver and subjected to N-terminal or matrix-assisted laser-desorption mass spectroscopic amino acid sequencing after tryptic in-gel digestion and high pressure liquid chromatography separation of the resulting peptides. Peptide sequences obtained were used to virtually screen expressed sequence tag data bases from human, mouse, and rat. Consensus cDNAs were assembled in silico from various expressed sequence tag sequences identified. Deduced mammalian protein sequences were characterized and compared with ribosomal protein sequences of Escherichia coli and yeast mitochondria. Significant sequence similarities to ribosomal proteins of other sources were detected for three out of four different mammalian protein classes determined. However, the sequence conservation between mitochondrial ribosomal proteins of mammalian and yeast origin is much less than the sequence conservation between cytoplasmic ribosomal proteins of the same species. In particular, this is shown for the mammalian counterparts of the E. coli EcoL2 ribosomal protein (MRP-L14), that do not conserve the specific and functional highly important His(229) residue of E. coli and the corresponding yeast mitochondrial Rml2p.
Keywords:Amino Acid Sequence, Bacterial Proteins, Escherichia Coli, Fungal Proteins, Mitochondria, Molecular Sequence Data, Protein Sequence Analysis, Ribosomal Proteins, Saccharomyces Cerevisiae, Sequence Alignment, Animals, Cattle, Mice, Rats
Source:Journal of Biological Chemistry
ISSN:0021-9258
Publisher:American Society for Biochemistry and Molecular Biology
Volume:274
Number:51
Page Range:36043-36051
Date:17 December 1999
Official Publication:http://www.jbc.org/cgi/content/abstract/274/51/36043
PubMed:View item in PubMed

Repository Staff Only: item control page

Open Access
MDC Library