Item Type: | Article |
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Title: | SMART, a simple modular architecture research tool: Identification of signaling domains |
Creators Name: | Schultz, J., Milpetz, F., Bork, P. and Ponting, C.P. |
Abstract: | Accurate multiple alignments of 86 domains that occur in signaling proteins have been constructed and used to provide a Web-based tool (SMART: simple modular architecture research tool) that allows rapid identification and annotation of signaling domain sequences. The majority of signaling proteins are multidomain in character with a considerable variety of domain combinations known. Comparison with established databases showed that 25% of our domain set could not be deduced from SwissProt and 41% could not be annotated by Pfam. SMART is able to determine the modular architectures of single sequences or genomes; application to the entire yeast genome revealed that at least 6.7% of its genes contain one or more signaling domains, approximately 350 greater than previously annotated. The process of constructing SMART predicted (i) novel domain homologues in unexpected locations such as band 4.1-homologous domains in focal adhesion kinases; (ii) previously unknown domain families, including a citron-homology domain; (iii) putative functions of domain families after identification of additional family members, for example, a ubiquitin-binding role for ubiquitin-associated domains (UBA); (iv) cellular roles for proteins, such predicted DEATH domains in netrin receptors further implicating these molecules in axonal guidance; (v) signaling domains in known disease genes such as SPRY domains in both marenostrin/pyrin and Midline 1; (vi) domains in unexpected phylogenetic contexts such as diacylglycerol kinase homologues in yeast and bacteria; and (vii) likely protein misclassifications exemplified by a predicted pleckstrin homology domain in a Candida albicans protein, previously described as an integrin. |
Keywords: | Computer Analysis, Diacylglycerol Kinases, DEATH Domain, Disease Genes, Automatic Sequence Annotation, Animals |
Source: | Proceedings of the National Academy of Sciences of the United States of America |
ISSN: | 0027-8424 |
Publisher: | National Academy of Sciences |
Volume: | 95 |
Number: | 11 |
Page Range: | 5857-5864 |
Date: | 26 May 1998 |
Official Publication: | http://www.pnas.org/content/95/11/5857.long |
PubMed: | View item in PubMed |
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