Effect of interleukin-10 on the production of tumor necrosis factor-alpha by peripheral blood mononuclear cells from patients with chronic heart failure

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Item Type:	Article
Title:	Effect of interleukin-10 on the production of tumor necrosis factor-alpha by peripheral blood mononuclear cells from patients with chronic heart failure
Creators Name:	Bolger, A.P., Sharma, R., von Haehling, S., Doehner, W., Oliver, B., Rauchhaus, M., Coats, A.J.S., Adcock, I.M. and Anker, S.D.
Abstract:	Chronic heart failure (HF) is a state of inflammatory immune activation characterized by elevated circulating levels of tumor necrosis factor-{alpha} (TNF-{alpha}). Interleukin-10 (IL-10) is a potent anti-inflammatory cytokine that inhibits TNF-{alpha} production and lessens endotoxin bioactivity. It is not known whether IL-10 reduces lipopolysaccharide (LPS) stimulated TNF-{alphaduction of peripheral blood mononuclear cells (PBMCs) from patients with chronic HF. PBMCs were isolated from 15 patients with chronic HF (New York Heart Association functional class 3.0 ± 0.2, left ventricular ejection fraction 30 ± 2%, peak oxygen consumption 18.1 ± 0.8 ml/kg/min) and 15 healthy control subjects and stimulated with 1 and 10 ng/ml LPS for 24 hours with or without prior addition of IL-10 (10 ng/ml). TNF-{alpha} was quantified in cell-free supernatants by an enzyme-linked immunosorbent assay. TNF-{alpha}, soluble TNF receptors, IL-10, and LPS were quantified in plasma. LPS stimulated TNF-{alpha} production was highest in those patients in New York Heart Association class II (p <0.01 vs New York Heart Association class III and IV, p <0.001 vs control subjects). IL-10 reduced PBMC TNF-{alpha} production in all stimulated samples at 1 and 10 ng/ml LPS (mean reduction 43% at 1 ng/ml, p <0.01 and 55% at 10 ng/ml, p <0.0001). The percentage reduction in TNF-{alpha} did not differ significantly between patients and control subjects or with respect to severity of chronic HF or baseline immune parameters. Independently of clinical severity, IL-10 profoundly inhibits TNF-α release from PBMCs isolated from patients with chronic HF. IL-10 is, therefore, a potential therapy for use in chronic HF associated with inflammatory immune activation.
Keywords:	Analysis of Variance, Congestive Heart Failure, Cultured Cells, Interleukin-10, Lipopolysaccharides, Mononuclear Leukocytes, Regression Analysis, Tumor Necrosis Factor-Alpha
Source:	American Journal of Cardiology
ISSN:	0002-9149
Publisher:	Elsevier
Volume:	90
Number:	4
Page Range:	384-389
Date:	1 January 2002
Official Publication:	https://doi.org/10.1016/S0002-9149(02)02494-3
PubMed:	View item in PubMed

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