Helmholtz Gemeinschaft

Search
Browse
Statistics
Feeds

Importin provides a link between nuclear protein import and U snRNA export

Item Type:Article
Title:Importin provides a link between nuclear protein import and U snRNA export
Creators Name:Goerlich, D., Kraft, R., Kostka, S., Vogel, F., Hartmann, E., Laskey, R.A., Mattaj, I.W. and Izaurralde, E.
Abstract:Importin-alpha mediates nuclear protein import by binding nuclear localization signals and importin-beta. We find approximately 30% of SRP1p, the yeast importin-alpha, in a nuclear complex with the Saccharomyces cerevisiae nuclear cap-binding protein complex (CBC). Similarly, a large fraction of Xenopus CBC is associated with importin-alpha in the nucleus. CBC promotes nuclear export of capped U snRNAs and shuttles between nucleus and cytoplasm. The CBC-importin-alpha complex binds specifically to capped RNA, suggesting that CBC might shuttle while bound to importin-alpha. Strikingly, importin-beta binding displaces the RNA from the CBC-importin-alpha complex. Thus, the commitment of CBC for nuclear reentry triggers the release of the export substrate into the cytoplasm. We provide evidence for a mechanism that ensures that importin-mediated RNA release is a specifically cytoplasmic event.
Keywords:alpha Karyopherins, Amino Acid Sequence, Biological Transport, Cell Nucleus, Cytoplasm, Fungal Genes, Guanosine Triphosphate, Karyopherins, Molecular Sequence Data, Nuclear Proteins, Oocytes, Protein Binding, ran GTP-Binding Protein, RNA Cap-Binding Proteins, RNA Caps, RNA-Binding Proteins, Saccharomyces Cerevisiae, Small Nuclear RNA, Animals, Xenopus
Source:Cell
ISSN:0092-8674
Publisher:Cell Press
Volume:87
Number:1
Page Range:21-32
Date:4 October 1996
Official Publication:https://doi.org/10.1016/S0092-8674(00)81319-7
PubMed:View item in PubMed

Repository Staff Only: item control page

Open Access
MDC Library