Functional interaction of beta-catenin with the transcription factor LEF-1

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Item Type:	Article
Title:	Functional interaction of beta-catenin with the transcription factor LEF-1
Creators Name:	Behrens, J., von Kries, J.P., Kuehl, M., Bruhn, L., Wedlich, D., Grosschedl, R. and Birchmeier, W.
Abstract:	The cytoplasmic proteins beta-catenin of vertebrates and armadillo of Drosophila have two functions: they link the cadherin cell-adhesion molecules to the cytoskeleton, and they participate in the wnt/wingless signal pathway. Here we show, in a yeast two-hybrid screen, that the architectural transcription factor LEF-1 (for lymphoid enhancer-binding factor) interacts with beta-catenin. In mammalian cells, coexpressed LEF-1 and beta-catenin form a complex that is localized to the nucleus and can be detected by immunoprecipitation. Moreover, LEF-1 and beta-catenin form a ternary complex with DNA that splays an altered DNA bend. Microinjection of LEF-1 into XenoPus embryos induces axis duplication, which is augmented by interaction with beta-catenin. Thus beta-catenin regulates gene expression by direct interaction with transcription factors such as LEF-1, providing a molecular mechanism for the transmission of signals, from cell-adhesion components or wnt protein to the nucleus.
Keywords:	beta Catenin, Cadherins, Cell Line, Cell Nucleus, Cytoskeletal Proteins, DNA, DNA-Binding Proteins, Escherichia Coli, Lymphoid Enhancer-Binding Factor 1, Messenger RNA, Molecular Cloning, Nucleic Acid Conformation, Protein Binding, Recombinant Proteins, Saccharomyces Cerevisiae, Trans-Activators, Transcription Factors, Xenopus Proteins, Animals, Drosophila, Xenopus
Source:	Nature
ISSN:	0028-0836
Publisher:	Nature Publishing Group
Volume:	382
Number:	6592
Page Range:	638-642
Date:	15 August 1996
Official Publication:	https://doi.org/10.1038/382638a0
PubMed:	View item in PubMed

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