Structure of the chicken myelomonocytic growth factor gene and specific activation of its promoter in avian myelomonocytic cells by protein kinases

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Item Type:	Article
Title:	Structure of the chicken myelomonocytic growth factor gene and specific activation of its promoter in avian myelomonocytic cells by protein kinases
Creators Name:	Sterneck, E., Blattner, C., Graf, T. and Leutz, A.
Abstract:	In chicken myeloid cells but not in erythroid cells, kinase-type oncogenes activate expression of the chicken myelomonocytic growth factor (cMGF). The autocrine loop established this way plays a key role in lineage-specific cooperation of nuclear and kinase-type oncogenes in retrovirally induced myeloid leukemia. In this report, we describe the cloning of the cMGF gene, including its promoter. The structure of the cMGF gene is homologous to those of the granulocyte colony-stimulating factor and interleukin-6 genes. Expression from reporter constructs containing the cMGF promoter is specific to myelomonocytic cells. Kinases activate cMGF at the transcriptional level in macrophages and strongly induce reporter expression in myelomonocytic cells.
Keywords:	Amino Acid Sequence, Avian Proteins, Base Sequence, Transformed Cell Line, Chromosome Mapping, Molecular Cloning, Neoplastic Gene Expression Regulation, Growth Substances, Intercellular Signaling Peptides and Proteins, Experimental Leukemia, Myeloid Leukemia, Molecular Sequence Data, Genetic Promoter Regions, Protein Kinases, Recombinant Fusion Proteins, Genetic Transcription, Animals, Chickens
Source:	Molecular and Cellular Biology
ISSN:	0270-7306
Publisher:	American Society for Microbiology
Volume:	12
Number:	4
Page Range:	1728-1735
Date:	April 1992
Official Publication:	https://doi.org/10.1128/mcb.12.4.1728-1735.1992
PubMed:	View item in PubMed

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