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| Item Type: | Article |
|---|---|
| Title: | Deuterated oxazines are bright near-infrared fluorophores for mitochondrial imaging and single molecule spectroscopy |
| Creators: |
Gatin-Fraudet, B.  ORCID: https://orcid.org/0000-0002-9000-9820, Seifert, J., Sarach, K., Olesen, C.H. ORCID: https://orcid.org/0000-0002-0575-1597, Birke, R., Milles, S. ORCID: https://orcid.org/0000-0001-9362-9606, Birol, M. ORCID: https://orcid.org/0000-0003-3397-8446, Lehmann, M. and Broichhagen, J. ORCID: https://orcid.org/0000-0003-3084-6595
|
| Abstract: | Bright near-infrared fluorophores are in demand for microscopy. We showcase a deuterated oxazine being 23% brighter vs. ATTO700. With a longer lifetime of 1.85 nanoseconds, we find the best-in-class SulfoOxazine700-d10 to stain mitochondria for confocal microscopy, and demonstrate unaffected diffusion properties in single molecule fluorescence correlation spectroscopy. |
| Keywords: | Confocal Microscopy, Deuterium, Fluorescence Spectrometry, Fluorescent Dyes, HeLa Cells, Mitochondria, Molecular Structure, Oxazines, Single Molecule Imaging |
| Source: | Chemical Communications |
| ISSN: | 1359-7345 |
| Publisher: | Royal Society of Chemistry |
| Volume: | 61 |
| Number: | 17 |
| Page Range: | 3540-3543 |
| Date: | 20 February 2025 |
| Official Publication: | https://doi.org/10.1039/d4cc03807j |
| PubMed: | View item in PubMed |
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