Concurrent profiling of localized transcriptome and RNA dynamics in neurons by spatial SLAMseq

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Item Type:	Article
Title:	Concurrent profiling of localized transcriptome and RNA dynamics in neurons by spatial SLAMseq
Creators Name:	Dantsuji, S. and Chekulaeva, M.
Abstract:	The asymmetric distribution of RNA within a cell plays a pivotal biological role, ensuring the distinctive shapes and functionality of subcellular compartments. In neurons, these mechanisms are fundamental to cellular growth, synaptic plasticity, and information processing. To understand these mechanisms, diverse methods have been developed to analyze localized transcripts. Here, we outline our optimized method for measurement of mRNA half-lives in subcellular neuronal compartments-neurites, and cytoplasmic and nuclear fractions of cell bodies. We call this method spatial SLAMseq, as it combines SLAMseq with subcellular compartment separation techniques. Spatial SLAMseq facilitates the concurrent measurement of mRNA dynamics and steady-state RNA levels within neuronal subcellular compartments.
Keywords:	mRNA Localization, mRNA Stability, Local Translation, SLAMseq, High-Throughput Sequencing, Neuron, Animals
Source:	Methods in Molecular Biology
Series Name:	Methods in Molecular Biology
Title of Book:	mRNA decay : methods and protocols
ISSN:	1064-3745
ISBN:	978-1-0716-4175-0
Publisher:	Springer / Humana Press
Volume:	2863
Page Range:	297-317
Date:	2025
Official Publication:	https://doi.org/10.1007/978-1-0716-4176-7_18
PubMed:	View item in PubMed

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