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| Item Type: | Article |
|---|---|
| Title: | Transcriptomic mapping of the 5-HT receptor landscape |
| Creators Name: | de Filippo, R. and Schmitz, D. |
| Abstract: | Serotonin (5-HT) is crucial for regulating brain functions such as mood, sleep, and cognition. This study presents a comprehensive transcriptomic analysis of 5-HT receptors (Htrs) across ≈4 million cells in the adult mouse brain using single-cell RNA sequencing (scRNA-seq) data from the Allen Institute. We observed differential transcription patterns of all 14 Htr subtypes, revealing diverse prevalence and distribution across cell classes. Remarkably, we found that 65.84% of cells transcribe RNA of at least one Htr, with frequent co-transcription of multiple Htrs, underscoring the complexity of the 5-HT system even at the single-cell dimension. Leveraging a multiplexed error-robust fluorescence in situ hybridization (MERFISH) dataset provided by Harvard University of ≈10 million cells, we analyzed the spatial distribution of each Htr, confirming previous findings and uncovering novel transcription patterns. To aid in exploring Htr transcription, we provide an online interactive visualizer. |
| Keywords: | 5-HT Receptors, Transcriptomics, 5-HT, Serotonin, scRNA-seq, MERFISH, Animals, Mice |
| Source: | Patterns |
| ISSN: | 2666-3899 |
| Publisher: | Cell Press / Elsevier |
| Volume: | 5 |
| Number: | 10 |
| Page Range: | 101048 |
| Number of Pages: | 1 |
| Date: | 11 October 2024 |
| Official Publication: | https://doi.org/10.1016/j.patter.2024.101048 |
| PubMed: | View item in PubMed |
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