Eosinophils are required to suppress Th2 responses in Peyer's patches during intestinal infection by nematodes

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Item Type:	Article
Title:	Eosinophils are required to suppress Th2 responses in Peyer's patches during intestinal infection by nematodes
Creators Name:	Strandmark, J., Steinfelder, S., Berek, C., Kühl, A.A., Rausch, S. and Hartmann, S.
Abstract:	Infections with enteric nematodes result in systemic type 2 helper T (Th2) responses, expansion of immunoglobulin (Ig)G1 antibodies, and eosinophilia. Eosinophils have a supportive role in mucosal Th2 induction during airway hyperreactivity. Whether eosinophils affect the local T-cell and antibody response in the gut-associated lymphoid tissue during enteric infections is unknown. We infected eosinophil-deficient ΔdblGATA-1 mice with the Th2-inducing small intestinal nematode Heligmosomoides polygyrus and found that parasite fecundity was decreased in the absence of eosinophils. A lack of eosinophils resulted in significantly augmented expression of GATA-3 and IL-4 by CD4(+) T cells during acute infection, a finding strictly limited to Peyer's patches (PP). The increase in IL-4-producing cells in ΔdblGATA-1 mice was particularly evident within the CXCR5(+)PD-1(+) T-follicular helper cell population and was associated with a switch of germinal centre B cells to IgG1 production and elevated serum IgG1 levels. In contrast, infected wild-type mice had a modest IgG1 response in the PP, whereas successfully maintaining a population of IgA(+) germinal center B cells. Our results suggest a novel role for eosinophils during intestinal infection whereby they restrict IL-4 responses by follicular T helper cells and IgG1 class switching in the PP to ensure maintenance of local IgA production.
Keywords:	B-Lymphocytes, Cultured Cells, Eosinophils, GATA3 Transcription Factor, Immune Tolerance, Immunoglobulin A, Immunoglobulin G, Inbred BALB C Mice, Inbred C57BL Mice, Interleukin-4, Intestines, Knockout Mice, Nematospiroides Dubius, Peyer's Patches, Strongylida Infections, Th2 Cells, Animals, Mice
Source:	Mucosal Immunology
ISSN:	1933-0219
Publisher:	Nature Publishing Group
Volume:	10
Number:	3
Page Range:	661-672
Date:	May 2017
Official Publication:	https://doi.org/10.1038/mi.2016.93
PubMed:	View item in PubMed

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