Helmholtz Gemeinschaft

Search
Browse
Statistics
Feeds

The IgCAM BT-IgSF (IgSF11) is essential for connexin43-mediated astrocyte-astrocyte coupling in mice

[thumbnail of Original Article]
Preview
PDF (Original Article) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
7MB

Item Type:Article
Title:The IgCAM BT-IgSF (IgSF11) is essential for connexin43-mediated astrocyte-astrocyte coupling in mice
Creators Name:Pelz, L., Dossou, L., Kompier, N., Jüttner, R., Siemonsmeier, G., Meyer, N., Lowenstein, E.D., Lahmann, I., Kettenmann, H., Birchmeier, C. and Rathjen, F.G.
Abstract:The type I transmembrane protein BT-IgSF is predominantly localized in the brain and testes. It belongs to the coxsackievirus and adenovirus receptor subgroup of Ig cell adhesion proteins, which are hypothesized to regulate connexin expression or localization. Here, we studied the putative link between BT-IgSF and connexins in astrocytes, ependymal cells, and neurons of the mouse. Global knock-out of BT-IgSF caused an increase in the clustering of connexin43 (Gja1), but not of connexin30 (Gjb6), on astrocytes and ependymal cells. Additionally, knock-out animals displayed reduced expression levels of connexin43 protein in the cortex and hippocampus. Importantly, analysis of biocytin spread in hippocampal or cortical slices from mature mice of either sex revealed a decrease in astrocytic cell–cell coupling in the absence of BT-IgSF. Blocking either protein biosynthesis or proteolysis showed that the lysosomal pathway increased connexin43 degradation in astrocytes. Localization of connexin43 in subcellular compartments was not impaired in astrocytes of BT-IgSF mutants. In contrast to connexin43, the localization and expression of connexin36 (Gjd2) on neurons were not affected by the absence of BT-IgSF. Overall, our data indicate that the IgCAM BT-IgSF is essential for correct gap junction–mediated astrocyte–astrocyte cell communication.
Keywords:IgCAM, BT-IgSF, Gap Junctions, Connexin43, Astrocyte-Astrocyte Coupling, Animals, Mice
Source:eNeuro
ISSN:2373-2822
Publisher:Society for Neuroscience
Volume:11
Number:3
Page Range:ENEURO.0283-23.2024
Date:March 2024
Official Publication:https://doi.org/10.1523/eneuro.0283-23.2024
PubMed:View item in PubMed

Repository Staff Only: item control page

Downloads

Downloads per month over past year

Open Access
MDC Library