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Using PrISMa to reveal the interactome of the human claudins family

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Item Type:Article
Title:Using PrISMa to reveal the interactome of the human claudins family
Creators Name:Perez-Hernandez, D., Suarez-Artiles, L., Jones, M.S.O. and Dittmar, G.
Abstract:Here, we provide a protocol for the systematic screening of protein-protein interactions mediated by short linear motifs using the Protein Interaction Screen on a peptide Matrix (PrISMa) technique. We describe how to pull down interacting proteins in a parallelized manner and identify them by mass spectrometry. Finally, we describe a bioinformatic workflow necessary to identify highly probable interaction partners in the large-scale dataset. We describe the application of this method for the transient interactome of the claudin protein family.
Keywords:High-Throughput Screening, Protein Biochemistry, Proteomics, Mass Spectrometry
Source:STAR Protocols
ISSN:2666-1667
Publisher:Cell Press / Elsevier
Volume:4
Number:4
Page Range:102549
Date:15 December 2023
Official Publication:https://doi.org/10.1016/j.xpro.2023.102549
PubMed:View item in PubMed

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