Lyophilization of synthetic gene carriers

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Item Type:	Article
Title:	Lyophilization of synthetic gene carriers
Creators Name:	Kasper, J.C., Hedtrich, S. and Friess, W.
Abstract:	Lyophilization, also known as freeze drying, is a widely used method for stabilization, improvement of long-term storage stability, and simplification of handling of drugs and/or carrier systems. Lyophilization is time-consuming and energy-consuming, and hence optimized processes are required to avoid time loss and higher costs without compromising product stability. Beginning from the last decade, nonviral, synthetic carriers for gene delivery have been of increasing interest. However, these systems suffer from poor physical stability in aqueous solution or suspension. Hence, to ensure long-term storage stability lyophilization of the gene carrier systems is favored. This chapter gives an overview of the basic steps and troubleshooting for successful lyophilization of synthetic gene carriers. Furthermore, the required excipients and their mechanism of action are summarized.
Keywords:	Lyophilization, Freeze Drying, Synthetic Gene Carriers, Stabilization, Formulation, Long Term Stability
Source:	Methods in Molecular Biology
Series Name:	Methods in Molecular Biology
Title of Book:	Nanotechnology for nucleic acid delivery
ISSN:	1064-3745
ISBN:	978-1-4939-9092-4
Publisher:	Springer / Humana Press
Volume:	1943
Number:	2
Page Range:	211-225
Number of Pages:	406
Date:	2019
Official Publication:	https://doi.org/10.1007/978-1-4939-9092-4_14
PubMed:	View item in PubMed

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