Brown adipose tissue transcriptome unveils an important role of the Beta-alanine/alamandine receptor, MrgD, in metabolism

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Item Type:	Article
Title:	Brown adipose tissue transcriptome unveils an important role of the Beta-alanine/alamandine receptor, MrgD, in metabolism
Creators Name:	Cerri, G.C., Santos, S.H.S., Bader, M. and Dos Santos, R.A.S.
Abstract:	Alamandine is a recently described heptapeptide component of the renin-angiotensin system (RAS), and its effects are mediated by the receptor MrgD. RAS represents an important link between obesity and its consequences by directly modulating the thermogenesis and brown adipose tissue (BAT) function. The alamandine/MrgD metabolic effects and signaling remain unexplored. In this context, the main goal of the present study was to assess the metabolic consequences of MrgD genetic ablation in C57BL6/J mice by evaluating brown adipose tissue RNA sequencing. The main results showed that MrgD-KO mice have diminished brown adipose tissue and that a high-glucose diet (HG) decreased both circulating alamandine levels and MrgD expression in BAT from wild-type mice (WT). BAT transcriptome reveals that MrgD-KO HG mice regulated 45 genes, while WT HG mice regulated 1148 genes. MrgD-KO mice fed a standard diet (ST) compared with WT ST mice regulated 476 genes, of which 445 genes were downregulated. BAT uses the MrgD receptor to display a normal pattern of gene expression and to respond, like WT mice, to an HG diet. In conclusion, the MrgD signaling is important for the metabolic regulation and manutention of BAT functionality.
Keywords:	Brown Adipose Tissue, Inbred C57BL Mice, Oligopeptides, Thermogenesis, Transcriptome, beta-Alanine, Animals, Mice
Source:	Journal of nutritional biochemistry
ISSN:	0955-2863
Publisher:	Elsevier
Volume:	114
Page Range:	109268
Date:	April 2023
Official Publication:	https://doi.org/10.1016/j.jnutbio.2023.109268
PubMed:	View item in PubMed

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