Combining metabolic pulse labeling and quantitative proteomics to monitor protein synthesis upon viral infection

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Item Type:	Article
Title:	Combining metabolic pulse labeling and quantitative proteomics to monitor protein synthesis upon viral infection
Creators Name:	Bogdanow, B., Katsimani, N., Liu, F. and Selbach, M.
Abstract:	Viruses like influenza A virus (IAV) hijack host cells in order to replicate. To actively and abundantly synthesize viral proteins, they reprogram the cellular transcriptional and translational landscape. Here, we present a proteomic approach that allows us to quantify the differences in host and viral protein synthesis comparatively for different strains of IAV. The method is based on combining quantitative proteomics using stable isotope labelling by amino acids in cell culture (SILAC) and bioorthogonal labeling with methionine analogs. This methodology accurately quantifies synthesis of host and viral proteins with high temporal resolution and faithfully detects global changes in cellular translation capacity. It thus provides unique insights into the dynamics of protein synthesis as the infection progresses.
Keywords:	Influenza A Virus, Translation, Species Barrier, Quantitative Proteomics, Orthogonal, Labeling, BONCAT, Shutoff
Source:	Methods in Molecular Biology
Series Name:	Methods in Molecular Biology
Title of Book:	Virus-Host Interactions
ISSN:	1064-3745
ISBN:	978-1-0716-2897-3
Publisher:	Springer / Humana Press
Volume:	2610
Page Range:	149-165
Date:	2023
Official Publication:	https://doi.org/10.1007/978-1-0716-2895-9_13
PubMed:	View item in PubMed

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