Braveheart, a long noncoding RNA required for cardiovascular lineage commitment

Tools

Item Type:	Article
Title:	Braveheart, a long noncoding RNA required for cardiovascular lineage commitment
Creators Name:	Klattenhoff, C.A., Scheuermann, J.C., Surface, L.E., Bradley, R.K., Fields, P.A., Steinhauser, M.L., Ding, H., Butty, V.L., Torrey, L., Haas, S., Abo, R., Tabebordbar, M., Lee, R.T., Burge, C.B. and Boyer, L.A.
Abstract:	Long noncoding RNAs (lncRNAs) are often expressed in a development-specific manner, yet little is known about their roles in lineage commitment. Here, we identified Braveheart (Bvht), a heart-associated lncRNA in mouse. Using multiple embryonic stem cell (ESC) differentiation strategies, we show that Bvht is required for progression of nascent mesoderm toward a cardiac fate. We find that Bvht is necessary for activation of a core cardiovascular gene network and functions upstream of mesoderm posterior 1 (MesP1), a master regulator of a common multipotent cardiovascular progenitor. We also show that Bvht interacts with SUZ12, a component of polycomb-repressive complex 2 (PRC2), during cardiomyocyte differentiation, suggesting that Bvht mediates epigenetic regulation of cardiac commitment. Finally, we demonstrate a role for Bvht in maintaining cardiac fate in neonatal cardiomyocytes. Together, our work provides evidence for a long noncoding RNA with critical roles in the establishment of the cardiovascular lineage during mammalian development.
Keywords:	Basic Helix-Loop-Helix Transcription Factors, Cell Differentiation, Embryonic Stem Cells, Gene Regulatory Networks, Mesoderm, Cardiac Myocytes, Polycomb Repressive Complex 2, Long Noncoding RNA, Animals, Mice, Rats
Source:	Cell
ISSN:	0092-8674
Publisher:	Cell Press
Volume:	152
Number:	3
Page Range:	570-83
Date:	31 January 2013
Official Publication:	https://doi.org/10.1016/j.cell.2013.01.003
PubMed:	View item in PubMed

Repository Staff Only: item control page